AO080513000070 (450 aa) Molybdopterin biosynthesis enzyme MGLSYTEAIR LVEAEAYRKR NVFLSRAETC SIYAARGRIA RHTIYSPIST PRFDTSAMDG YALSSAATKD ATVEYPRTFE VKGITAAGDE PFTEVKHDGP IPPCVEIMTG APFPKTVEQE DFDCCVRYED VIVEERSGRR FVTVTKPAKP KQNRRLAATD FQTDNAIVKE GELIHPNQVM AMASVGITEL SVLLKPRIAV FSTGSELLLA KEESTKLHRI SDSNGPYLTA TLEDWGAIVD FLGVIPDQTE DLEQALLRGL HGSRYDLIIS SGAVSAGRYD LIPAVLERLR ARTVFHKVEM KPGHPVLFSM LPRLETAPDS EVAFFGLPGN PVASAACLRF IVVPYLNTIR LQPQEKPYKA RIMTMNDKLG SHHDIPLGSG KPVTTVPMEK DVFRPGTLCG STDQDLMVQL ISDHSPGKIK PFLEANCWIR IPHGVSEIRE GDSVGIYPMR
PSG: a new signal peptide prediction method N-region: length 10; pos.chg 1; neg.chg 1 H-region: length 2; peak value -10.87 PSG score: -15.27 GvH: von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -7.38 possible cleavage site: between 34 and 35 >>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: 1 Number of TMS(s) for threshold 0.5: 0 PERIPHERAL Likelihood = 1.91 (at 333) ALOM score: 0.37 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 1 Hyd Moment(75): 2.58 Hyd Moment(95): 4.38 G content: 1 D/E content: 2 S/T content: 2 Score: -6.70 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: RRFVTVTKPAKPKQNRR at 139 content of basic residues: 11.6% NLS Score: 0.02 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: none SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : MGLSYTE Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none checking 71 PROSITE ribosomal protein motifs: none checking 33 PROSITE prokaryotic DNA binding motifs: none NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 89 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
39.1 %: nuclear 34.8 %: cytoplasmic 13.0 %: mitochondrial 8.7 %: vesicles of secretory system 4.3 %: vacuolar >> prediction for AO080513000070 is nuc (k=23)