AO080525000577 (404 aa) Synaptic vesicle protein EHS-1 and related EH domain proteins MSNKKIEQWE IERYWEIFAS LANGQPRLNN SQAASVLRNS RLRDEQLEKV WDLADVDGDG ELDFEEFCVA MRLVFDLVNG ELQTVPAVLP DWLVPESKSH LVHATRALST QPEQFERIED EDDTPGLKDG FEWYMKPADK SKYEEIYNAN RNQRGEIAFE SLQPLYDSLD VPDTDVRSAW NLVNPSASHT INKDATLAFL HILNYRHEGF RIPRTVPASL RASFENNKID YQVDNARPAQ KWGADGDTET PTGRKTKFGD TYLSRLGAGG KSSYTPKGTN FSDTIQDEEW EKVRLRRELA ELETKLNSAQ QASEGRRDQP RNDGRTSWGL VKKEALQLLE YKERELRELR EGTGRSKEGQ NLERLREDVK AVGEQVDGLK SHLANRNEVL ADLRRQIDEE KVSR
PSG: a new signal peptide prediction method N-region: length 10; pos.chg 2; neg.chg 2 H-region: length 1; peak value -9.20 PSG score: -13.60 GvH: von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -7.78 possible cleavage site: between 24 and 25 >>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: 0 number of TMS(s) .. fixed PERIPHERAL Likelihood = 3.61 (at 62) ALOM score: 3.61 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 0 Hyd Moment(75): 8.83 Hyd Moment(95): 9.01 G content: 0 D/E content: 2 S/T content: 1 Score: -6.03 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: none pat7: PTGRKTK (3) at 251 bipartite: none content of basic residues: 14.6% NLS Score: -0.22 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: KKXX-like motif in the C-terminus: EKVS SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none checking 71 PROSITE ribosomal protein motifs: none checking 33 PROSITE prokaryotic DNA binding motifs: none NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: nuclear Reliability: 70.6 COIL: Lupas's algorithm to detect coiled-coil regions 290 E 0.97 291 K 0.97 292 V 0.97 293 R 0.97 294 L 0.97 295 R 0.97 296 R 0.97 297 E 0.97 298 L 0.97 299 A 0.97 300 E 0.97 301 L 0.97 302 E 0.97 303 T 0.97 304 K 0.97 305 L 0.97 306 N 0.97 307 S 0.97 308 A 0.97 309 Q 0.97 310 Q 0.97 311 A 0.97 312 S 0.97 313 E 0.97 314 G 0.97 315 R 0.97 316 R 0.97 317 D 0.97 318 Q 0.96 355 S 0.71 356 K 0.87 357 E 0.87 358 G 0.87 359 Q 0.87 360 N 0.87 361 L 0.87 362 E 0.87 363 R 0.87 364 L 0.87 365 R 0.87 366 E 0.87 367 D 0.87 368 V 0.87 369 K 0.87 370 A 0.87 371 V 0.87 372 G 0.87 373 E 0.94 374 Q 0.94 375 V 0.94 376 D 0.94 377 G 0.94 378 L 0.94 379 K 0.94 380 S 0.94 381 H 0.94 382 L 0.94 383 A 0.94 384 N 0.94 385 R 0.94 386 N 0.94 387 E 0.94 388 V 0.94 389 L 0.94 390 A 0.94 391 D 0.94 392 L 0.94 393 R 0.94 394 R 0.94 395 Q 0.94 396 I 0.94 397 D 0.94 398 E 0.94 399 E 0.94 400 K 0.94 401 V 0.66 total: 76 residues
78.3 %: nuclear 13.0 %: mitochondrial 8.7 %: cytoplasmic >> prediction for AO080525000577 is nuc (k=23)