AO080527000391 (304 aa) Transcription elongation factor TFIIS/Cofactor of enhancer-binding protein Sp1 MPLEAKEIEL KAKALTKAAT QNEPAANIVS LLKELQQGVK ATEDLLRSTR VGIIVNKFKQ HKSPEVSRLS SEIVSRWRNE VNKQKASGSP APSQRSSNSP RPAQNGTASP AGTTPTDKAS KLSVAPDKRT WKADGVDINQ TGNKIRDSCI GLMYDGLCLN STEAPRNVLS KASAVEAAAY KSLGPETKEQ YRTKIRSLFQ NLKNKSNPSL RIRVLSNDVT PDQFVRMSHD ELRSDEQREK DAKIQKENMD KAMVAQAERS ISTSLQCGKC GQRKVTYTEA QTRSADEPMT LFCTCLNCGK SWKQ
PSG: a new signal peptide prediction method N-region: length 11; pos.chg 2; neg.chg 3 H-region: length 1; peak value 0.00 PSG score: -4.40 GvH: von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -7.78 possible cleavage site: between 41 and 42 >>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: 0 number of TMS(s) .. fixed PERIPHERAL Likelihood = 9.12 (at 148) ALOM score: 9.12 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 0 Hyd Moment(75): 2.59 Hyd Moment(95): 6.04 G content: 0 D/E content: 2 S/T content: 0 Score: -7.54 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: none content of basic residues: 16.4% NLS Score: -0.47 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: KKXX-like motif in the C-terminus: KSWK SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none checking 71 PROSITE ribosomal protein motifs: none checking 33 PROSITE prokaryotic DNA binding motifs: none NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: nuclear Reliability: 94.1 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
82.6 %: nuclear 13.0 %: mitochondrial 4.3 %: peroxisomal >> prediction for AO080527000391 is nuc (k=23)