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AO080533000200 (102 aa) Predicted protein MLRIAVVIKW FKEARTGDLQ HIRSVLRQDP YNSGPGNDAA KLQAFSFFKN RLAPRMTSRK MNSSPAVLSA QPDKGEQRER QTMGKEDDFT NGNTGICMCF SG
PSG: a new signal peptide prediction method
N-region: length 9; pos.chg 2; neg.chg 0
H-region: length 2; peak value -10.41
PSG score: -14.81
GvH: von Heijne's method for signal seq. recognition
GvH score (threshold: -2.1): -7.48
possible cleavage site: between 16 and 17
>>> Seems to have no N-terminal signal peptide
ALOM: Klein et al's method for TM region allocation
Init position for calculation: 1
Tentative number of TMS(s) for the threshold 0.5: 0
number of TMS(s) .. fixed
PERIPHERAL Likelihood = 10.45 (at 1)
ALOM score: 10.45 (number of TMSs: 0)
MITDISC: discrimination of mitochondrial targeting seq
R content: 2 Hyd Moment(75): 6.92
Hyd Moment(95): 8.39 G content: 1
D/E content: 2 S/T content: 1
Score: -5.02
Gavel: prediction of cleavage sites for mitochondrial preseq
R-2 motif at 13 LRI|AV
NUCDISC: discrimination of nuclear localization signals
pat4: none
pat7: none
bipartite: none
content of basic residues: 15.7%
NLS Score: -0.47
KDEL: ER retention motif in the C-terminus: none
ER Membrane Retention Signals:
XXRR-like motif in the N-terminus: LRIA
none
SKL: peroxisomal targeting signal in the C-terminus: none
SKL2: 2nd peroxisomal targeting signal: none
VAC: possible vacuolar targeting motif: none
RNA-binding motif: none
Actinin-type actin-binding motif:
type 1: none
type 2: none
NMYR: N-myristoylation pattern : none
Prenylation motif: none
memYQRL: transport motif from cell surface to Golgi: none
Tyrosines in the tail: none
Dileucine motif in the tail: none
checking 63 PROSITE DNA binding motifs: none
checking 71 PROSITE ribosomal protein motifs: none
checking 33 PROSITE prokaryotic DNA binding motifs: none
NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination
Prediction: nuclear
Reliability: 94.1
COIL: Lupas's algorithm to detect coiled-coil regions
total: 0 residues
56.5 %: nuclear 43.5 %: mitochondrial >> prediction for AO080533000200 is nuc (k=23)