Content-type: text/html PSORT II server

Input Sequence

AO080533000200 (102 aa) Predicted protein

MLRIAVVIKW FKEARTGDLQ HIRSVLRQDP YNSGPGNDAA KLQAFSFFKN
RLAPRMTSRK MNSSPAVLSA QPDKGEQRER QTMGKEDDFT NGNTGICMCF
SG


Results of Subprograms

PSG:  a new signal peptide prediction method
      N-region:  length 9;  pos.chg 2;  neg.chg 0
      H-region:  length 2;  peak value -10.41
      PSG score: -14.81

GvH:  von Heijne's method for signal seq. recognition
      GvH score (threshold: -2.1):  -7.48
      possible cleavage site: between 16 and 17

>>> Seems to have no N-terminal signal peptide

ALOM: Klein et al's method for TM region allocation
      Init position for calculation: 1
      Tentative number of TMS(s) for the threshold  0.5:   0
      number of TMS(s) .. fixed
      PERIPHERAL  Likelihood = 10.45 (at 1)
      ALOM score:  10.45  (number of TMSs: 0)

MITDISC: discrimination of mitochondrial targeting seq
      R content:       2       Hyd Moment(75):  6.92
      Hyd Moment(95):  8.39    G content:       1
      D/E content:     2       S/T content:     1
      Score: -5.02

Gavel: prediction of cleavage sites for mitochondrial preseq
      R-2 motif at 13  LRI|AV

NUCDISC: discrimination of nuclear localization signals
      pat4: none
      pat7: none
      bipartite: none
      content of basic residues:  15.7%
      NLS Score: -0.47

KDEL: ER retention motif in the C-terminus: none

ER Membrane Retention Signals: 
      XXRR-like motif in the N-terminus: LRIA

none

SKL: peroxisomal targeting signal in the C-terminus: none

SKL2: 2nd peroxisomal targeting signal:  none

VAC: possible vacuolar targeting motif: none

RNA-binding motif: none

Actinin-type actin-binding motif:
      type 1: none
      type 2: none

NMYR: N-myristoylation pattern : none

Prenylation motif: none

memYQRL: transport motif from cell surface to Golgi: none

Tyrosines in the tail: none

Dileucine motif in the tail: none

checking 63 PROSITE DNA binding motifs:  none

checking 71 PROSITE ribosomal protein motifs:  none

checking 33 PROSITE prokaryotic DNA binding motifs:  none

NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination
      Prediction: nuclear
      Reliability: 94.1

COIL: Lupas's algorithm to detect coiled-coil regions
      total: 0 residues 


Results of the k-NN Prediction

k = 9/23
	 56.5 %: nuclear
	 43.5 %: mitochondrial

>> prediction for AO080533000200 is nuc (k=23)