AO080536000083 (218 aa) Predicted protein MGILLTEEEI ETVTPIVKPC YAALGLANDY FSFDVEWKEF QERQSDEGAM TNAVWLFMQW NNVDVATAKK LVWEVTNRYE EEYQRRVEEF IAGEGRNAAK LHTYLRALAY QIPGNIAWSL RCPRYHPELC DEAGRLLEDE MCSETATNVL GHPVEHERGS NTTGSEKSPV WTADDNSSKR SSVSSIDAIE EEAESPKPEQ LGTEVGIFAI HHSRCPAD
PSG: a new signal peptide prediction method N-region: length 11; pos.chg 0; neg.chg 4 H-region: length 6; peak value 0.00 PSG score: -4.40 GvH: von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -9.40 possible cleavage site: between 27 and 28 >>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: 0 number of TMS(s) .. fixed PERIPHERAL Likelihood = 5.04 (at 10) ALOM score: 5.04 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 0 Hyd Moment(75): 7.94 Hyd Moment(95): 6.25 G content: 1 D/E content: 2 S/T content: 1 Score: -6.95 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: none content of basic residues: 9.2% NLS Score: -0.47 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: none SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : MGILLTE Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none checking 71 PROSITE ribosomal protein motifs: none checking 33 PROSITE prokaryotic DNA binding motifs: none NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 89 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
65.2 %: cytoplasmic 26.1 %: nuclear 4.3 %: Golgi 4.3 %: mitochondrial >> prediction for AO080536000083 is cyt (k=23)