AO080554000065 (310 aa) Geranylgeranyl pyrophosphate synthase/Polyprenyl synthetase MQALEAWLRD RGLQEAFEKI LTGPLDYLRE NPGKDIRAKL IDAFNEFLEV PNDKLDVIKR IIDLLHNASL LIDDIQNSST LRRGVPVAHS IFGVAQTINS ANYAYFIAQR ELTNLTNPLS FSIYTEELIH LHRGQGMELH WRDTMQCPSE VEYIEMALDK TGGLFRLAIR LMQAESASGT DYVPLVETLG LLFQIRDDYQ NLQSDTYSTN KGFCEDIGEG KFSYPIIHSI RSRPGDLRLL SILKQRSEDI TVRKYAVEYI DSTGSFDYCE RKIISLMQQA REQVRRSIAD TTHRGSQIEK ILNMLEIDKK
PSG: a new signal peptide prediction method N-region: length 11; pos.chg 2; neg.chg 2 H-region: length 3; peak value -10.56 PSG score: -14.96 GvH: von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -12.86 possible cleavage site: between 14 and 15 >>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: 0 number of TMS(s) .. fixed PERIPHERAL Likelihood = 5.62 (at 56) ALOM score: 5.62 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 1 Hyd Moment(75): 4.19 Hyd Moment(95): 6.75 G content: 0 D/E content: 2 S/T content: 0 Score: -6.32 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: none content of basic residues: 11.9% NLS Score: -0.47 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: KKXX-like motif in the C-terminus: EIDK SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none checking 71 PROSITE ribosomal protein motifs: none checking 33 PROSITE prokaryotic DNA binding motifs: none NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 55.5 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
43.5 %: cytoplasmic 34.8 %: nuclear 17.4 %: mitochondrial 4.3 %: vesicles of secretory system >> prediction for AO080554000065 is cyt (k=23)