AO080568000198 (475 aa) Ribosomal protein S6 kinase and related proteins MGNSQGKPVC STDEINLNQF RLLRVVGKGA FGKVRIVEKK DTGLTFALKY IRKEEGIIHR DVKPDNVLLD SEGHVHLADF NVASDFRPGK PLTSKSGTLA YLAPEVYEGG GYYCEVDWWS LGVTFYECIY NKRPFEGRSQ DVLSENIKKA QPKYYVTNPA VSVPCLRAMG ALLEKDRSKR IGASSWESFI SHIFFAEIDF VALEHKEIPP VFRPSSDKTN FDATYDLEEL LLEEAPLEAR ARRQKPRAEL REDATAKEIR EDELHRLIET MFEPFDYTTV TFQGNPEDCL PPAGSTHSRQ YSQTDSSRNS PPLRNNDGSV SRLTQPETPS PLGETVDHQD SIPNQAPTSP TSHPQPPPSP APQFHRPFHP QPNPAANHLP PANSNPAPGR PKGATRKTSK GGGVQMVLEE AGSWSELADQ SSTLPAEGYD AGSIKGKSSN SGMLAFLSRK KGRDRSPKPQ EPGVLGKEGA RQIIS
PSG: a new signal peptide prediction method N-region: length 7; pos.chg 1; neg.chg 0 H-region: length 5; peak value -4.22 PSG score: -8.62 GvH: von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -6.37 possible cleavage site: between 32 and 33 >>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: 0 number of TMS(s) .. fixed PERIPHERAL Likelihood = 3.13 (at 187) ALOM score: 3.13 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 0 Hyd Moment(75): 4.95 Hyd Moment(95): 6.58 G content: 2 D/E content: 2 S/T content: 3 Score: -7.20 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: none content of basic residues: 12.4% NLS Score: -0.47 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: none SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : MGNSQGK Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none checking 71 PROSITE ribosomal protein motifs: none checking 33 PROSITE prokaryotic DNA binding motifs: none NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: nuclear Reliability: 94.1 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
69.6 %: nuclear 13.0 %: cytoplasmic 13.0 %: mitochondrial 4.3 %: peroxisomal >> prediction for AO080568000198 is nuc (k=23)